Journal of Marine Biology & Oceanography ISSN: 2324-8661

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Research Article, J Mar Biol Oceanogr Vol: 2 Issue: 3

On the Optimization of Hydrolysis Conditions for Simultaneous Determination of Amino Acids and Amino Sugars in Marine Sediments

Santiago Priotto1* and Rubén J. Lara1,2
1Instituto Argentino de Oceanografía, Florida 4750, 8000 Bahía Blanca,Argentina
2Leibniz Centre for Tropical Marine Ecology, Fahrenheitstr. 6, 28359 Bremen,Germany
Corresponding author : Santiago Priotto
Instituto Argentino de Oceanografía, Florida 4750, 8000 Bahía Blanca, Argentina
E-mail: [email protected]
Received: May 27, 2013 Accepted: July 12, 2013 Published: July 18, 2013
Citation: Priotto S, Lara RJ (2013) On the Optimization of Hydrolysis Conditions for Simultaneous Determination of Amino Acids and Amino Sugars in Marine Sediments. J Mar Biol Oceanogr 2:3. doi:10.4172/2324-8661.1000112

Abstract

On the Optimization of Hydrolysis Conditions for Simultaneous Determination of Amino Acids and Amino Sugars in Marine Sediments

In the present work different approaches were tested for the optimization of the hydrolysis conditions for the joint determination of amino acids and amino sugars in chitin, an important structural polymer in fungi, invertebrates, algae and the exoskeleton of zooplankton. Ten treatments were evaluated, involving different combinations of hydrolysis temperature and duration. The obtained results indicate that slightly lower temperatures (105ºC) than for the classical combined amino acid hydrolysis and longer times (22 hours) than for chitin minimized losses and maximized recoveries of monomeric amino acids and amino sugars.

 

Keywords: Amino acids; Amino sugars; Sediments; Hydrolysis; Methodological optimization; Temperature treatment

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