Research Article, J Mar Biol Oceanogr Vol: 7 Issue: 1
Composition of Docosahexaenoic Acid, Eicosapentaenoic Acid and Palmitoleic Acid in Eucheuma denticulatum, Sargassum polycystum and Caulerpa lentillifera Ethanolic Extracts by LC-ESI-MS/MS
Vimala B1,5*, June Chelyn L2, Brownlee IA3, Mohd Fairunizal MN1, Syahida A4 and Amin I5
1Nutrition Unit, Cardiovascular, Diabetes and Nutrition Research Centre, Institute for Medical Research, Jalan Pahang, 50588 Kuala Lumpur, Malaysia
2Phytochemistry Unit, Herbal Medicine Research Centre, Institute for Medical Research, Jalan Pahang, 50588 Kuala Lumpur, Malaysia
3Human Nutrition Research Centre, Newcastle University, 172A Ang Mo Kio, Avenue 8, 567739, Singapore
4Faculty of Biotechnology and Biomolecular Sciences, University Putra Malaysia, 43400 Serdang, Selangor, Malaysiaa
5Department of Nutrition and Dietetics, Faculty of Medicine and Health Sciences, University Putra Malaysia, 43400 Serdang, Selangor, Malaysia
*Corresponding Author : Vimala Balasubramaniam
Nutrition Unit, Cardiovascular, Diabetes and Nutrition Research Centre, Institute for Medical Research, Jalan Pahang, 50588 Kuala Lumpur, Malaysia
Tel: +603-26162635/+60129693294
Fax: +603-26939335
E-mail: balavimala@hotmail.com
Received: November 03, 2017 Accepted: January 06, 2018 Published: January 12, 2018
Citation: Vimala B, June Chelyn L, Brownlee IA, Mohd Fairunizal MN, Syahida A, et al. (2018) Composition of Docosahexaenoic Acid, Eicosapentaenoic Acid and Palmitoleic Acid in Eucheuma denticulatum, Sargassum polycystum and Caulerpa lentillifera Ethanolic Extracts by LC-ESI-MS/MS. J Mar Biol Oceanogr 7:1. doi: 10.4172/2324-8661.1000185
Abstract
Composition of Docosahexaenoic Acid, Eicosapentaenoic Acid and Palmitoleic Acid in Eucheuma denticulatum, Sargassum polycystum and Caulerpa lentillifera Ethanolic Extracts by LC-ESI-MS/MS
Omega-3 and omega-7 fatty acids have been linked to health benefit in humans. Seaweeds are potential source of fatty acids. Thus, in the current study, a rapid and highly selective method with minimal sample preparation based on liquid chromatographic electrospray ionization tandem mass spectrometry (LC/MS/MS) has been developed and validated for the quantitative determination of docosahexaenoic acid (DHA), eicosapentaenoic acid (EPA) and palmitoleic acid (PA) in Eucheuma denticulatum, Caulerpa lentillifera and Sargassum polycystum ethanolic extracts. The levels of DHA, EPA and PA were in ranges of 6.0-7.0 μg/100 g DW, 51.0- 122.0 μg/100 g DW and 5.0-690.0 μg/100 g DW respectively in the three species of seaweed tested. The standard calibration curves for PA, DHA and EPA were linear (R2 > 0.99) over the concentration ranged between 0-3.00 ng μL-1, respectively, satisfactory recoveries (> 77%), low detection (0.0047- 0.0175 ng μL-1) and quantification limits (0.0156-0.0580 ng μL-1) (LOD and LOQ, respectively). These results indicated that LC-ESI-MS/MS was successfully applied to the analysis of the selected fatty acids in the three groups of seaweeds.
