VEGETOS: An International Journal of Plant ResearchOnline ISSN: 2229-4473
Print ISSN: 0970-4078

Research Article, Vegetos Vol: 30 Issue: 4

Gibberellins Production Techniques by Immobilization

Ahamad S1*, Sharma SK1 and Agarwal DK2

1Directorate of Research, Sher-e-Kashmir University of Agricultural Sciences and Technology of Jammu (J and K), India

2Division of Plant Pathology, IARI, New Delhi, India

*Corresponding Author : Ahamad S
Directorate of Research, Sher-e-Kashmir University of Agricultural Sciences and Technology of Jammu (J and K), India
Tel: 7889808422
E-mail: [email protected]

Received: May 08, 2017 Accepted: August 08, 2017 Published: August 10, 2017

Citation: Ahamad S, Sharma SK, Agarwal DK (2017) Gibberellins Production Techniques by Immobilization. Vegetos 30:4.doi: 10.4172/2229-4473.1000317

Abstract

The gibberellins are one of the major groups of growth promoting hormones which play an essential role in regulation of growth and development of angiospermic plants. Gibberellins are secondary metabolites of the fungus, Gibberella fujiluroi with its imperfect state of Fusarium moniliforme. F. moniliforme gives higher yields of GA3 on a variety of media and is associated with bakane diseases of rice. The performance of immobilized growing cells of Gibbrella fujikuroi-52 was affected by the immobilization agent used, nature and age of cells, mycelial cell density and size of beads. The beads, prepared by using standardized procedures, could be used for 7 cycles without affecting productivity in semi-continuous culture. The rate of production of gibberellic acid was 0.33-65 mg/l/h. In immobilized cells, GF-52 produced highest GA3 i.e. 0.99 g/l as compared to 0.77 g from in un-immobilized cells. Immobilized cells produced 0.74 g/l as compared to 0.57 g/l in un-immobilized cells.

Keywords: GA3; Gibberellic acid; Gibberella fujikuroi; Immobilization; Fusarium monilifurme

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