Journal of Plant Physiology & Pathology ISSN: 2329-955X

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Research Article, J Plant Physiol Pathol Vol: 4 Issue: 1

Induction of Glutathione, Ascorbate and Associated Enzymes by a Low Dose CdCl2 Pre-treatment Alleviate Fusarium Induced Oxidative Stress in Wheat

Subhalaxmi Mohapatra and Bhabatosh Mittra*
Department of Biosciences and Biotechnology, Fakir Mohan University, Vyasa Vihar, Balasore-756020, Odisha, India
Corresponding author : Bhabatosh Mittra
Department of Biosciences and Biotechnology, Fakir Mohan University, Vyasa Vihar, Balasore-756020, Odisha, India
Tel: 91-9938984421
Fax: 06782-2354881
E-mail: [email protected]
Received: June 18, 2015 Accepted: January 21, 2016 Published: January 26, 2016
Citation: Mohapatra S, Mittra B (2016) Induction of Glutathione, Ascorbate and Associated Enzymes by a Low Dose CdCl2 Pre-treatment Alleviate Fusarium Induced Oxidative Stress in Wheat. J Plant Physiol Pathol 4:1. doi:10.4172/2329-955X.1000141

Abstract

Induction of glutathione (GSH), ascorbate (ASC) as well as their associated enzymes have been investigated in co-stressed (50 μM CdCl2 pre-treatment followed by Fusarium infection) wheat
seedlings
. An enhanced GSH content, GSH/GSSG ratio and ASC content was observed in co-stressed tissues as compared to Fusarium infected tissues. Further, co-stressed tissues showed an increased activity of the antioxidant enzymes such as ascorbate peroxidase (APX) and glutathione reductase (GR) involved in glutathione-ascorbate cycle as well as glutathione peroxidase (GSHPx) and glutathione-S-transferase (GST) as glutathione associated enzymes when compared with Fusarium infected, Cd2+ pre-treated and untreated control tissues. In contrast, a decline in hydrogen peroxide (H2O2) and oxidised glutathione (GSSG) content was observed in co-stressed tissues as compared to Fusarium infected
tissues. Thus, a low dose (50 μM) Cd2+ pre-treatment induced both the enzymatic and non-enzymatic antioxidants to maintain redox state at cellular level. Induction of these enzymes is suggested to
be a part of defence system and play important role in offering resistance against Fusarium infection in wheat.

Keywords: Ascorbate; Fusarium oxysporum; GSH; GSSG; Low dose Cadmium; Triticum aestivum

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