Journal of Plant Physiology & Pathology ISSN: 2329-955X

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Research Article, J Plant Physiol Pathol Vol: 7 Issue: 1

Leaf Variegation Alters in Antioxidant Activity and Phenolic CompoundsConcentration of Indian Borage (Plectranthus amboinicus)

Jean Carlos Vencioneck Dutra1*, Paula Roberta Costalonga Pereira1, Polianna da Silva Ferreira2, Juliana Macedo Delarmelina3, Claudia Masrouah Jamal4 and Maria do Carmo Pimentel Batitucci1

1Laboratório de Genética Vegetal e Toxicológica, Departamento de Ciências Biológicas, Universidade Federal do Espírito Santo, 29075-910, Vitória, Espírito Santo, Brazil

2Instituto Federal do Espírito Santo – Campus Vila Velha, 29106-010, Vila Velha, Espírito Santo, Brazil

3Instituto Federal do Espírito Santo – Campus São Mateus, 29932-540, São Mateus, Espírito Santo, Brazil

4Laboratório de Química de Produtos Naturais, Departamento de Ciências Farmacêuticas – Universidade Federal do Espírito Santo, 29040-090, Vitória, Espírito Santo, Brazil

*Corresponding Author : Jean Carlos Vencioneck Dutra
Laboratório de Genética
Vegetal e Toxicológica, Departamento de Ciências Biológicas, Universidade
Federal do Espírito Santo, 29075-910, Vitória, Espírito Santo, Brazil, E-mail:
[email protected]

Received: March 06, 2019 Accepted: March 15, 2019 Published: March 22, 2019

Citation: Dutra JCV, Pereira PRC, Ferreira PS, Delarmelina JM, Jamal CM, et al. (2019) Leaf Variegation Alters in Antioxidant Activity and Phenolic Compounds Concentration of Indian Borage (Plectranthus amboinicus). J Plant Physiol Pathol 7:1. doi: 10.4172/2329-955X.1000194

Abstract

Plectranthus amboinicus (Indian borage) is used in folk medicine and culinary and presented a variegated version. This work aimed to evaluate the influence leaf variegation on chemical composition and biological activities of P. amboinicus and P. amboinicus “Variegata”. Hydroalcoholic extracts of herbs were evaluated for their preliminary phytochemistry; total flavonoid and rosmarinic acid content by spectrophotometry and HPLC methods, respectively; antioxidant activity on DPPH, ABTS, Fe2+ chelation ions and β-carotene/linoleic acid assays; and antiproliferative effects in saroma 180 cells by MTT assay. Preliminary phytochemistry showed that both plants present flavonoids, flavone and coumarins. P. amboinicus “Variegata” extract presented higher levels of flavonoid and rosmarinic acid and higher antioxidant activity in DPPH and ABTS assays, as well as P. amboinicus extract showed better antioxidant activity in the Fe2+ chelating activity and inhibition of β-carotene oxidation. Both extracts induced pronounced anticancer effects on sarcoma 180. We hypothesize that variegation promotes modulations in secondary metabolism, which leads to the increase of total flavonoids and rosmarin acid biosynthesis, interfering in the biological activities exhibited for P. amboinicus extracts.

Keywords: Antioxidant activity; Indian borage; MTT assay; Phytochemistry; Sarcoma 180 anticancer

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