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Andrology & Gynecology: Current ResearchISSN: 2327-4360

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Research Article, Androl Gynecol Curr Res Vol: 4 Issue: 3

Lindane Induces Spermatotoxicity and inhibits Steroidogenesis in Adult Rats

Hamdy AA Aly1,2*, Abdulrahman M Alahdal3, Alaa Bagalagel3 Ahmed M Mansour2, Abdel-Moneim M Osman4, Ibrahim A Shehata5, and Waleed El-Shaer 6
1Department of Pharmacology and Toxicology, Faculty of Pharmacy, King Abdulaziz University, Jeddah, Saudi Arabia
2Department of Pharmacology and Toxicology, Faculty of Pharmacy, Al-Azhar University, Nasr City, Cairo, Egypt
3Department of Clinical Pharmacy, Faculty of Pharmacy, King Abdulaziz University, Jeddah, Saudi Arabia
4Department of Pharmacology, College of Medicine, King Abdulaziz University, Jeddah, Saudi Arabia
5Department of Natural Products and Alternative Medicine, Faculty of Pharmacy, King Abdulaziz University, Jeddah, Saudi Arabia
6Departments of urology, Banha University Hospital, Egypt
Corresponding author : Hamdy AA Aly
Department of Pharmacology and Toxicology, Faculty of Pharmacy, King Abdulaziz University, Jeddah, Saudi Arabia
Tel:
+966 0542497023
E-mail: [email protected]
Received: January 13, 2016 Accepted: October 25, 2016 Published: November 01, 2016
Citation: Hamdy AA Aly, Abdulrahman M. Alahdal, Alaa Bagalagel, Mansour AM, Osman AM, Shehata IA, El-Shaer W (2016) Lindane Induces Spermatotoxicity and Inhibits Steroidogenesis in Adult Rats. Androl Gynecol: Curr Res 4:2. doi: 10.4172/2327-4360.1000150

Abstract

This study was conducted to investigate the influence of lindane on spermatogenesis and testicular biochemical parameters and to get insight into its mechanism. Adult male albino rats were treated orally with lindane at doses of 0, 1, 2 or 4 mg/kg/day for 30 consecutive days. Testes weight and sperm count and motility were significantly decreased. Testicular activities of 3β-HSD, 17β-HSD were significantly inhibited. Testicular cholesterol level was significantly increased while glycogen and sialic acid content were significantly decreased. Testicular activities of LDH-X, γ-GT, β-glucuronidase and acid phosphatase (ACP) were significantly decreased. Aldo-ketoreductase activity was significantly decreased in response to 2 or 4 mg/kg/day of lindane while, protein carbonyl contentswere significantly increased. Hydrogen peroxide and hydroxyl radical generation and LPO were significantly increased. The enzymatic antioxidants SOD, CAT and GPx and the non-enzymatic antioxidants GSH and Vit C were significantly decreased. Lindane at doses 2 and 4 mg/kg/day showed a significant reduction in Vit E, while at a dose of 1 mg/kg/day did not show any significant change. In conclusion, lindane inhibits spermatogenesis, steroidogenesis and suppresses Sertoli cell marker enzymes which may be due, partly, to oxidative stress. Lindane enhances ROS generation and LPO and depletes antioxidant enzymes and non-enzymatic antioxidants.

Keywords: Lindane; Testis; Spermatogenesis; Steroidogenesis; Oxidative stres

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