VEGETOS: An International Journal of Plant ResearchOnline ISSN: 2229-4473
Print ISSN: 0970-4078

Research Article, Vegetos Vol: 30 Issue: 1

Marker Assisted Selection for Bacterial Leaf Blight resistance in Segregating Populations of Karma Mahsuri

Umesh Chandra Deshmukh1*, Ravindra Kumar Verma1, Ritu R Saxena1, Pratibha Mohan2 and Satish B Verulkar2
1Department of Genetics and Plant Breeding, Indira Gandhi Krishi Vishwavidyalaya, Raipur, 492012, Chhattisgarh, India
2Department of Plant Molecular Biology and Biotechnology, Indira Gandhi Krishi Vishwavidyalaya, Raipur, 492012, Chhattisgarh, India
Corresponding author : Umesh Chandra Deshmukh
Department of Genetics and Plant Breeding, Indira Gandhi Krishi Vishwavidyalaya, Raipur, 492012, Chhattisgarh, India E-mail: [email protected]
Received: December 27, 2016 Accepted: January 21, 2017 Published: January 24, 2017
Citation: Deshmukh UC, Verma RK, Saxena RR, Mohan P, Verulkar SB (2017) Marker Assisted Selection for Bacterial Leaf Blight resistance in Segregating Populations of Karma Mahsuri. Vegetos 30:1. doi: 10.5958/2229-4473.2017.00010.6

Abstract

Marker Assisted Selection for Bacterial Leaf Blight resistance in Segregating Populations of Karma Mahsuri

Bacterial leaf blight (BLB) of rice is caused by Xanthomonas oryzae pv. oryzae (Xoo), this is a major disease of rice in several countries. The present investigation was undertaken with the objective to develop high yielding rice varieties possessing broad spectrum durable resistance by transferring bacterial leaf blight (BLB) resistant genes viz., xa5 xa13 and Xa21 from a donor IRBB59 and a popular high yielding rice variety i.e., Karma Mahsuri, susceptible to BLB and were selected as parents. The presence of target genes by using gene linked primers viz., xa5R, xa5S, xa13 promoter and Xa21F/R was done. Segregating population was advanced to BC2 F3 generation and foreground selection was done using gene linked markers. Genetic analysis in BC2 F3 populations confirmed the presence of genes (xa5, xa13 & Xa21) governing BLB resistance in 22 lines. p>

Keywords: Rice; Bacterial leaf blight; Background selection; Foreground selection; Marker assisted selection; Segregating population

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