Andrology & Gynecology: Current ResearchISSN: 2327-4360

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Research Article, Androl Gynecol Curr Res Vol: 1 Issue: 3

Microfluidic Device Increases Sperm Concentration by Optimization of Chamber Structure

Koji Matsuura1,2*, Ken Nishida3, Ikuyo Sugimoto1 and Shinichiro Yanase3
1Research Core for Interdisciplinary Sciences, Okayama University, Okayama, Japan
2Cardiovascular Physiology, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University, Okayama, Japan
3Graduate School of Natural Science and Technology, Okayama University, Okayama, Japan
Corresponding author : Koji Matsuura, PhD
Lecturer, Cardiovascular Physiology, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University, Okayama, 700-8530, Japan
Tel: +81-86-251-8966; Fax: +81-86-251-8966; E-mail: [email protected]
Received: March 11, 2013 Accepted: June 13, 2013 Published: June 18, 2013
Citation: Matsuura K, Nishida K, Sugimoto I, Yanase S (2013) Microfluidic Device Increases Sperm Concentration by Optimization of Chamber Structure. Androl Gynecol: Curr Res 1:3. doi:10.4172/2327-4360.1000106

Abstract

Microfluidic Device Increases Sperm Concentration by Optimization of Chamber Structure

Intracytoplasmic sperm injection (ICSI) is an extremely timeconsuming and tedious procedure in cases of oligozoospermia, because it is difficult to obtain a sufficient number of motile sperms. To circumvent this difficulty, we previously developed a microfluidic device to increase sperm concentration and reduce time required for ICSI. Here, we improved this device by carefully optimizing its dimensions. We achieved a 10–13-fold enrichment ratio in non-motile human sperms (1.0 × 104 cells/ml) when a chamber height of 1.4 mm was used, and we described a mathematical model to explain the relationship between sperm concentration and enrichment ratio. However, this model was unable to explain the decrease in the enrichment ratio when a chamber height of 1 mm was used.

Keywords: Male subfertility; Oligozoospermia; In vitro fertilization; Intracytoplasmic sperm injection; Microfluidic device

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