Research Journal of Zoology

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Short Communication, Research Journal Of Zoology Vol: 3 Issue: 4

Mouse model of oral acute infection with Trypanosoma cruzi reveals inflammatory changes and hemostatic derangement

Dina Antunes
 

Oswaldo Cruz Foundation, Rio de Janeiro, Brazil

Abstract

Statament of the Problem: Oral transmission of Trypanosoma cruzi, the etiologic agent of Chagas disease, is the most important route of infection in Brazilian Amazon. Other South American countries have also reported outbreaks associated with food consumption. This route of transmission presents specific symptoms such as facial and lower limbs edema and, in some cases, bleeding and thrombosis. Nevertheless, studies that address this transmission are largely lacking regarding its pathogenesis and, more specifically, the crosstalk between immune and hemostatic systems. Methodology: BALB/c mice, aged 6±8 weeks, were infected via oral cavity (Oral infection) with 5x104 metacyclic trypomastigotes Tulahuén strain, obtained from excreta of Triatoma infestans. Animals were handled according to the rules of the Ethics Committee for Animal Research of Oswaldo Cruz Foundation. Findings: When compared with control uninfected animals, orally infected mice presented high proinflammatory cytokine (TNF-α, IFN-γ and IL-6) serum levels. The highest concentrations were obtained concomitantly to the peak of parasitemia. Blood counts revealed thrombocytopenia in the oral infected group at 14 and 21 days post-infection (dpi) and leukocytosis at 21 dpi. Tail bleeding assay displayed that animals bled more at 21 dpi. Coagulation factor concentrations varied during infection with an increase in Factor V and Factor VIII consumption at 14 dpi indicating disturbances in the coagulation cascade. Activated partial thromboplastin time (aPTT) and Prothrombin time (PT) tests corroborated the hematological changes with an increase in clotting time at 21 dpi. Moreover, preliminary data indicate amelioration of the orally infected animals after blockage of IL-6 receptor, revealing a critical role of IL-6 in the course of oral infection. Conclusion & Significance: These results demonstrate, for the first time, that T. cruzi oral infection results in significant alterations in hemostatic system and indicates the relevance of the crosstalk between inflammation and hemostasis in this parasitic disease

Keywords: Zoology

Statament of the Problem: Oral transmission of Trypanosoma cruzi, the etiologic agent of Chagas disease, is the most important route of infection in Brazilian Amazon. Other South American countries have also reported outbreaks associated with food consumption. This route of transmission presents specific symptoms such as facial and lower limbs edema and, in some cases, bleeding and thrombosis. Nevertheless, studies that address this transmission are largely lacking regarding its pathogenesis and, more specifically, the crosstalk between immune and hemostatic systems. Methodology: BALB/c mice, aged 6±8 weeks, were infected via oral cavity (Oral infection) with 5x104 metacyclic trypomastigotes Tulahuén strain, obtained from excreta of Triatoma infestans. Animals were handled according to the rules of the Ethics Committee for Animal Research of Oswaldo Cruz Foundation. Findings: When compared with control uninfected animals, orally infected mice presented high proinflammatory cytokine (TNF-α, IFN-γ and IL-6) serum levels. The highest concentrations were obtained concomitantly to the peak of parasitemia. Blood counts revealed thrombocytopenia in the oral infected group at 14 and 21 days post-infection (dpi) and leukocytosis at 21 dpi. Tail bleeding assay displayed that animals bled more at 21 dpi. Coagulation factor concentrations varied during infection with an increase in Factor V and Factor VIII consumption at 14 dpi indicating disturbances in the coagulation cascade. Activated partial thromboplastin time (aPTT) and Prothrombin time (PT) tests corroborated the hematological changes with an increase in clotting time at 21 dpi. Moreover, preliminary data indicate amelioration of the orally infected animals after blockage of IL-6 receptor, revealing a critical role of IL-6 in the course of oral infection. Conclusion & Significance: These results demonstrate, for the first time, that T. cruzi oral infection results in significant alterations in hemostatic system and indicates the relevance of the crosstalk between inflammation and hemostasis in this parasitic disease.

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