Research Article, J Mar Biol Oceanogr Vol: 5 Issue: 2
Short-Term Effects of CO2-Induced Low pH Exposure on Target Gene Expression in Platynereis dumerilii
|Janine Wäge1,2, Silvia Rohr3, Jörg D Hardege1 and Jeanette M Rotchell1*|
|1School of Biological, Biomedical and Environmental Sciences, University of Hull, Cottingham Road, Hull, HU6 7RX, United Kingdom|
|2Leibniz Institute for Baltic Sea Research Warnemünde (IOW), Seestrasse 15, 18119 Rostock, Germany|
|3European Molecular Biology Laboratory, Heidelberg, Meyerhofstr. 1, 69117 Heidelberg, Germany|
|Corresponding author : Jeanette M Rotchell
School of Biological, Biomedical and Environmental Sciences, University of Hull, Cottingham Road, Hull, HU6 7RX, United Kingdom
Tel: +44 (0)1482 465333
Fax: +44 (0)1482 465458
|Received: May 24, 2016 Accepted: June 29, 2016 Published: July 05, 2016|
|Citation: Wäge J, Rohr S, Hardege JD, Rotchell JM (2016) Short-Term Effects of CO2-Induced Low pH Exposure on Target Gene Expression in Platynereis dumerilii. J Mar Biol Oceanogr 5:2. doi:10.4172/2324-8661.1000155|
Short-Term Effects of CO2- Induced Low pH Exposure on Target Gene Expression in Platynereis dumerilii
Objective: Increasing atmospheric CO2 concentration are causing changes to the seawater carbonate chemistry, lowering the pH and we study potential impacts of these changes at the molecular level in a non-calcifying, marine polychaete species Platynereis dumerilii.
Methods: We investigate the relative expression of carbonic anhydrase (CA), Na+/H+ exchangers (NHE), and calmodulin (CaM) genes from P. dumerilii under acidified seawater conditions (pH 7.8) induced by CO2 using qPCR.
Results: mRNA expression of CA in the CO2-induced worms was significantly up-regulated at low pH conditions (pH 7.8, 1h), suggesting changes in acid-base balance. In contrast, the expression of NHE and CaM showed no significant change. In addition, we compare these results to a previous study using inorganic acid (HCl)-induced pH changes.
Conclusions: Results suggest that carbonate chemistry has an impact on gene expression that differs from pH-associated change. To our knowledge, this is the first study that compares low pH exposure experiments using HCl and CO2 as the inducing agents.