Antimicrobial property of crude Butanol, Dichloromethane, and Hexane leaf extracts of Wrightia antidysenterica

Ang Dean Kyle T, Avena Glormina A, Balisi Julianne Patricia G, Benin Bianca Marie B, Cabezon Nicholaire C, Clavio Roy Isaac D F and Lacson Mary Roussle
Janthina C

Antimicrobial property of crude Butanol, Dichloromethane, and Hexane leaf extracts of Wrightia antidysenterica

Ang Dean Kyle T, Avena Glormina A, Balisi Julianne Patricia G, Benin Bianca Marie B, Cabezon Nicholaire C, Clavio Roy Isaac D F and Lacson Mary Roussle Janthina C

University of Santo Tomas Faculty of Pharmacy, Philippines

: J Pharm Drug Deliv Res

Abstract

Wrightia antidysenterica, belonging to family Apocynaceae, is commonly grown in lowlands of the country. Several species of the same family were reported to have medicinal properties. The research analyzes the antimicrobial properties of crude butanol, dichloromethane (DCM) and hexane leaf extracts of Wrightia antidysenterica. Extracts were tested against significant strains of microorganisms: Staphylococcus aureus, Staphylococcus epidermidis, and genera Bacillus, Escherichia, Psuedomonas, Micrococcus, Streptococcus, Klebsiella, Proteus, Salmonella, and Candida. Zones of inhibition in the agar disc diffusion method revealed that S. pneumoniae (14.40 mm) and S. epidermidis (15.70 mm) are susceptible to DCM leaf extracts. The antibiotic activity of this leaf extracts is attributed to the phytochemicals: steroids, tannins, phenols, alkaloids, saponins, coumarins, and terpenoids. The two-fold serial dilution determined that at a concentration of 100 mg/ml, the DCM leaf extract possessed antibiotic activity comparable to gentamicin against S. pneumoniae and S. epidermidis. This was recorded as the MIC. The activity of 100 mg/ml is observed to be only bacteriostatic upon streaking and 24-incubation on MHA; whereas, activity of gentamicin remained bactericidal against the two species of bacteria. Therefore, Wrightia antidysenterica cannot be used as an alternative bactericidal agent against S. epidermidis and S. pneumoniae. However, it can be used as a bacteriostatic agent against these two species of bacteria.