Capparis spinosa L. fruits aqueous extract improves insulin resistance in streptozotocin-induced diabetic mice
Hebi M, Ajebli M, EL Bouhali B, Benaji B, Issad N and Eddouks M
Faculty of Sciences and Tecniques Errachidia, Moulay Ismail University
Faculty of Sciences and Techniques Mohammadia, Moroco
Faculty of Sciences Ben Msik, Casablanca, Morocco
: J Pharm Drug Deliv Res
Abstract
Majority of diabetic patients make recourse to medicinal plants/herbal-based remedies as alternative therapies to diabetes mellitus. Capparis spinosa L. (CS) is a medicinal plant used in the traditional medicinal for the treatment of diabetes mellitus, however, the mechanism of action involved in this pharmacological property of this plant remains undetermined. This study was undertaken in order to evaluate the effect of aqueous CS extract on insulin resistance in diabetic mice. Both single and repeated oral administrations of aqueous CS extract were performed multi-low dose streptozotocin-induced (MLDS) diabetic mice. In addition, in order to determine the effect of aqueous CN extract on insulin resistance, euglycemic hyperinsulinemic clamp has been performed and the endogenous glucose production has been analysed using a perfusion of perfusion of 3-3H glucose. Our present study has shown that aqueous CS extract evoked a potent hypoglycaemic activity in MLDS diabetic mice. In other hand, perfusion of 3-3H glucose demonstrated that this hypoglycaemic activity was accompanied by a decrease in basal endogenous glucose production (EGP). EGP was lower in CS-Treated group when compared to the control group, 17.5±2.4 vs 27.2±7.1 mg/kg.min-1 (p<0.001) respectively. Using the euglycemic hyperinsulinemic clamp technique, the study demonstrated that CS treatment also improves insulin sensitivity in peripheral tissues. We conclude that the hypoglycaemic activity of aqueous CS extract is due, at least in part, to the inhibition of basal endogenous glucose production and the improvement of peripheral insulin resistance in MLDS diabetic mice.
Biography
Email: mohamed.eddouks@laposte.net
