Cardiovascular effect of Nigella sativa L. aqueous extract in normal rats
Hebi M, Zeggwagh N-A., Benaji B, Michel JB and Eddouks
Faculty of Sciences and Techniques Errachidia, BP.21, Moulay Ismail University, 52000, Morocco
U698 INSERM, Faculty of Medicine Xavier Bichat, Paris, France
Department of Biology, Faculty of Sciences Ain Chock, Casablanca
: J Pharm Drug Deliv Res
Abstract
The present wok aims to evaluate the cardiovascular effect of Nigella sativa L. aqueous extract (NSAE) in normal rats. The in vivo experiment showed that the intravenous injection of NSAE at the doses of 50, 100 and 200 mg/kg of body weight produced a dose dependent reduction in the mean arterial blood pressure (MABP) (p<0.001) accompanied by a significant fall in heart rate (p<0.01). In the in vitro experiment, NSAE was tested at the doses of 10, 20 and 30 mg/ml. Addition of NSAE to the plateau contraction induced by Norepinephrine (NE) produced a dose dependent reduction in the arterial tone (p<0.01). Furthermore, incubation of NSAE during 30 min caused a right shift of the contraction response curve of aortic ring to NE with a reduction of the maximal contraction response (p<0.01). Endothelium destruction significantly reduced the vasorelaxant effect of NSAE at a dose of 30 mg/ml (p<0.01). Furthermore, Nitric oxide synthase inhibitor: Nï·-Nitro-LArgenine Methyl (L-NAME) produced a significant reduction (p<0.01) of the in vitro vasorelaxant effect of NSAE at a dose of 30 mg/ml. We conclude that NSAE possess a rapid and dose dependent in vivo hypotensive effect in normal rats which may be probably due to the inhibition of parasympathetic tone. In isolated aortic ring, NSAE possess a potent inhibitor of contractile response to NE which may be probably due to an increase in the endothelial nitric oxide synthesis. This work was supported by the CNRST under grant N° PPR/2015/35.
Biography
Email: mohamed.eddouks@laposte.net
