Chemiluminescence mediated light free photodynamic therapy

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Berwin Singh S V, Dileep R T and Dongwon Lee

Chonbuk National University, Republic of Korea

: J Pharm Drug Deliv Res

Abstract

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Statement of the Problem: Photodynamic Therapy (PDT) is a treatment modality that employs an extrinsic photosensitive dye that could mediate a photochemical reaction involving conversion of molecular oxygen from triplet state to singlet in the presence of photons of suitable wavelength. However, penetration of the photons to the tumor interstitium is a major concern that limits PDT in majority of the biomedical application. At present, several researchers have developed “self-illuminating” PDT systems, in which photosensitizers are excited by Chemiluminescent Resonance Energy Transfer (CRET) or bioluminescent resonance energy transfer (BRET). In this work, we had demonstrated the use of hydroxybenzyl alcohol-incorporating copolyoxalate peroxalate ester loaded protoporphyrin nano-self-assembly (CDNP) that could mediate Chemiluminasence Dynamic Therapy (CDT). Methodology & Theoretical Orientation: CDNP react with HPOX to generate 1,2-dioxethanedione as an intermediate of high energy. Highly unstable 1,2-dioxethanedione rapidly decomposes and releases energy which could activate PPIX to generate 1O2 that ultimately kill the cancer cells: we termed this POCL dependent event as CDT. Findings: We successfully developed CDNP with average diameter of 200 nm. Morphological assessment, flow cytometric analysis, western blotting and confocal fluorescence micrographs indicates CDNP co-treatment with CA exhibited significant cytotoxicity in SW620 cells (human colorectal adenocarcinoma). Conclusion & Significance: We have validated that CDNP could induce 1O2 in the presence of H2O2 without any external photon to exert highly potent anticancer activity. This work provides for the first time, a proof of concept for laser-free PDT as a novel anticancer therapeutic system. We anticipate that CDT could provide solutions to the challenges in PDT and hold great potential as a promising cancer treatment modality.

Biography

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Berwin Singh S V is a PhD student at the Chonbuk National University in Republic of Korea working under the supervision of Prof. Dongwon Lee. He received his Master’s in Bio Medical Science from the Bharathidhasan University, India, later he was trained in Bio Medical Technology (MPhil) at the Sri Chitra Instititute of Medical Science, India. His current research is focused on smart polymer synthesis-physiochemical characterization, developing and evaluation of nano-carriers in vitro and in vivo.

Email: berwinsingh1989@jbnu.ac.kr