Core shell technology: New trends in ultra fast HPLC
Imran Ali
Jamia Millia Islamia, India
: J Diagnos Tech Biomed Anal
Abstract
Core shell technology is gaining importance in HPLC analyses due to economic, fast and reproducible nature. Core shell columns are called as new generation columns. Various brand names are available with Sunshell, Halo, Ascentis Express, Poroshell 120, Kinetex, Accucore and Nucleoshell trade names. These columns provide ultra fast HPLC separations for a variety of phramaceuticals with moderate sample loading, capacity and low back pressure. Chemistries of these columns are C8, C18, RP Amide, Hilic, PFP, Phenyl, and RP-aqua as well WP C 18. Silica particles are of 2.6 and 1.6 μm as total and inner solid core diameters with 0.5 µm thickness of outer porous layer; having 90 Å pore sizes and 150 m2/g surface areas. The proposed lecture will high light the importance of new generation columns with special emphasis on their textures and chemistries, separations, optimization and comparison (inter and intra stationary phases) and economy. Besides, future perspectives will also be discussed.
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