Determination of critical micelle concentration of lipid based systems with Cisplatin
Irfan Akartas, Yildirim Y, Ak G, Karasulu E, Sanlier S and Karasulu H Y
Ege University, Turkey
: J Pharm Drug Deliv Res
Surfactant solutions which are prepared by different concentrations could show rapid changes of its osmotic pressure, conductivity, turbidity and surface tension at higher concentrations. McBain associated these rapid changes with the formation of micelles or aggregates. The lipophilic hydrocarbon chains which have hydrophilic groups that interact with water phase move to the inner part of the micelles. The critical micelle concentration (CMC) is defined as the concentration of surfactants above which micelles form and all additional surfactants added to the system go to micelles. The aim of this study is determination of critical micelle concentration of lipid based system with cisplatin by surface tension method. Isopropyl myristate was used as the oil phase, kolliphor were used as the surfactant, and propylene glycol was used as the co-surfactant to produce lipid based system. Surface tension is typically measured in dynes/cm, the force in dynes required to break a film of length 1 cm. There are several methods to measure the surface tension such as Wilhelmy plate, capillary rise method, and drop weight method. In this study, traube stalagmometer is used for drop weight method. Water and HCl buffer with pH 1, 2 and phosphate buffer with pH 6, 8 were used as solvents. 0.24x10-3, 1.2x10-3, 2x10-3, 4x10-3 and 6x10-3 g/ml concentrations of SMEDDS with cisplatin are prepared to measure surface tension in these solvents.