Development and applications of desorption atmospheric pressure photoionization-mass spectrometry
Jan RejÅ¡ek, VladimÃÂr Vrkoslav and Josef CvaÄÂka
Institute of Organic Chemistry and Biochemistry, Czech Republic, Charles University in Prague, Czech Republic
: J Diagnos Tech Biomed Anal
Abstract
D esorption atmospheric pressure photoionization (DAPPI) is the ionization technique examined in this study. This method employs solvent spray for desorption and ionization of analytes from the solid surfaces. The aim of this study was to develop a rapid analytical technique enabling to localize separated compounds on the TLC plate and secretory gland openings on the insect body surface and characterize compounds of the secretion using a setup combining DAPPI-MS with a software-controlled moveable motorized sample holder. Six lipid standards and then vernix caseosa extract were separated on TLC plates. Developed TLC plates were mounted on software-controlled moveable motorized sample holder, and spatial distribution of separated compounds was tracked. Soldiers of the termite Prorhinotermes simplex and adult stink bugs Graphosoma lineatum were killed by freezing and fixed on the glass slide using the correction fluid. Body surface was scanned by means of DAPPI to map the spatial distribution of defense compounds. DAPPI in negative ion mode was used to map the spatial distribution of ( E )-1-nitropentadec-1-ene on the body surface of P. simplex soldier. Opening of the frontal gland was localized. DAPPI in positive ion mode was used to track the spatial distribution of selected unsaturated aldehydes on the body surface of G. lineatum . Opening of the metathoracic scent glands was localized in the posterior part of the thorax. Effect of surface roughness was evaluated and limit of detection was calculated In brief, development of non-destructive imaging technique suitable for the examination of TLC plates and biological samples was done
Biography
Jan Rejšek graduated from Charles University in Prague in analytical chemistry. Now, he is doing his PhD on ambient mass spectrometry at the Institute of Organic Chemistry and Biochemistry, Academy of Sciences of the Czech Republic. He worked for three years in the State Veterinary Institute Prague in the laboratory of atomic spectrometry. He is specialized in the development and routine use of spectrometric analytical techniques.
