Development of a suppositories pilot batch of leaves and stems of Artemisia annua grown in Cameroon
Pierre Lutgen, Chougouo Kengne R D, Domko D F, Kouamouo J, Djoko E, Tane P and Wouessidjewe D
IFBV-BELHERB, Luxembourg
Université des Montagnes, Cameroon
University of Dschang, Cameroon
University of Joseph Fourier of Grenoble, France
: J Pharm Drug Deliv Res
Abstract
Malaria is the most deadly disease that concerns mostly African children under the age of 5. It is a difficult treatment because of drug resistance to conventional molecules leads to the use of Artemisinin-based combination therapy (ACT) recommended by WHO. Several studies showed that the infusion of A. annua is more efficient than ACT after 7 days of treatment, but hardly accepted by children mostly those less than 2 years old because of the quantity to administration. The present study is to put in place a more acceptable dosage form for children suppositories made from A. annua grown in Cameroon. To evaluate its quality, the powder of leaves and stems of A. annua has been submitted to physicochemical analysis. The particle size was isolated by the sieve method and laser diffraction. Artemisinin was determined by TLC-densitometry and then was read through MESURIM software. Entire flavonoids were titrated by aluminum chloride. The formula of medicines was established and suppositories were submitted for pharmacotechnical tests. The powder obtained was of bitter taste, greyish-green and with characteristic odor (camphor), was homogeneous with 56.37% of particles in the sieve of diameter over or equal to 63 μm. The artemisinin and entire flavonoids contents were respectively of 5 mg/g and 0.43 mg equivalent to quercetin per gram of dry matter. 250 mg suppositories of active principle have been made knowing that, 1g of A. annua powder moves 0.72 g of Suppocire C. They are dark-green, shiny, smooth and barrelshaped. Their average weight was 2.15 g, disintegration time was 8 min 16 s and the fusion point was 35.7 oC. The made A. annua suppositories were in conformity with European pharmacopoeia. The suppositories will contribute to a better treatment of malaria among children.
Biography
Email: lutgenp@gmail.com
