Effect of micafungin on biofilm of Candida dubliniensis isolated from oral cavity of HIV-infected patients

Seyyed Amin Ayatollahi Mousavi

Effect of micafungin on biofilm of Candida dubliniensis isolated from oral cavity of HIV-infected patients

Seyyed Amin Ayatollahi Mousavi

Kerman University of Medical Sciences, Iran

: J Pharm Drug Deliv Res

Abstract

Candida dubliniensis is opportunistic yeast that has been recently implicated in oropharyngeal candidiasis in human immunodeficiency virus-infected patients. Cells within the biofilm communities display a phenotype resistant to antimicrobials and host defences, so biofilm-associated infections are difficult to treat, representing a source of reinfections. The present study evaluated the effect of Micafungin on the biofilm formation capacity of C. dubliniensis isolated from the oral cavity of HIV-infected patients. MICs were determined using broth microdilution according to the CLSI reference method M27-A2 (3). RPMI 1640 was used as the test medium and we used the MIC endpoint of prominent growth reduction at 24 h. We determined the MICs of micafungin against 120 human immunodeficiency virus-infected patients of Nystatin & fluconazole-resistant. Micafungin had good in vitro activity against the drug-resistant C. dubliensis tested; the MICs at which 50% (MIC (50)) and 90% (MIC (90)) of isolates were inhibited were 0.03microg/ml and 0.06microg/ml, respectively. The drugresistant C. dubliensis was inhibited at a micafungin MIC that was