Electronic label - Free biosensing assays
Mark A Reed
Yale University, USA
: J Electr Eng Electron Technol
Abstract
Nanoscale electronic devices have the potential to achieve exquisite sensitivity as sensors for the direct detection of molecular interactions, thereby decreasing diagnostics costs and enabling previously impossible sensing in disparate field environments. Semiconducting nanowire field effect transistors (NW-FETs) hold particular promise, though contemporary NW approaches are inadequate for realistic applications and integrated assays. We present here an integrated nanodevice biosensor approach that is compatible with CMOS technology, has achieved unprecedented sensitivity, and simultaneously facilitates system-scale integration of nanosensors. These approaches enable a wide range of label-free biochemical and macromolecule sensing applications, such as specific protein and complementary DNA recognition assays, and specific macromolecule interactions at femtomolar concentrations. Critical limitations of nanowire sensors are the Debye screening limitation, and the lack of internal calibration for analyte quantification, which has prevented their use in clinical applications and physiologically relevant solutions. We will present approaches that solve these longstanding problems, which demonstrate the detection at clinically important concentrations of biomarkers from whole blood samples, integrated assays of cancer biomarkers, and the use of these as a quantitative tool for drug design and discovery, including binding kinetics and chirality detection.
Biography
Mark A Reed holds the Harold Hodgkinson Chair of Engineering and Applied Science at Yale University, and is the Associate Director of the Yale Institute for Nanoscience and Quantum Engineering. He is the author of more than 180 professional publications and 6 books, has given over 25 plenary and over 360 invited talks. His awards include the Kilby Young Innovator Award, the Fujitsu ISCS Quantum Device Award, Fellow of the American Physical Society, Fellow of the Institute of Electrical and Electronics Engineers, and the IEEE Pioneer Award in Nanotechnology.
