Enantiomeric separation of biologically active 2-Pyrazolines on Lux Cellulose-2 and Lux Amylose-2: A comparative study
Mohammed Farrag El-Behairy and Aida El-Azzouny
National Research Centre, Egypt
: J Diagnos Tech Biomed Anal
Abstract
The enantioselective resolution of a set of biologically active 3-(benzo[d][1,3]dioxol-5-yl)-5-tert-butyl-4,5-dihydropyrazole derivatives (1-21) is demonstrated. Two chiral stationary phases based on the chloromethylphenylcarbamate derivatives of amylose or cellulose: namely Lux Cellulose-2 and Lux Amylose-2, were evaluated for the chiral separation of theses 2-pyrazolines using enantioselective high performance liquid chromatography (HPLC). Different mobile phases and separation modes have been investigated supported by the versatility of Lux columns. Baseline separations of all investigated compounds have been achieved using different mobile phases such as (pure Methanol, Acetonitrile, or Ethanol, or Mixtures of Methanol/Acetonitrile, Acetonitrile/Ethanol, Hexane/Ethanol). In general, Cellulose-2 column showed better enantio-separation for most of the compounds represented by high resolution in shorter time. For example compound 4 (R = H) showed Rs = 20 and run time 36 min when using Acetonitrile 100% on lux cellulose-2 while using Methanol/Acetonitrile 1/1 v/v showed Rs = 8 and run time up to 6.0 min (Rs = 1.40 for amylose-2).
Biography
Mohammed F. El-Behairy has completed his BSc of pharmacy on 2003 with grade excellent honors and PhD at the age of 29 years from Faculty of Pharmacy, Cairo University as joint supervision between NRC, Egypt and NTNU, Norway and postdoctoral studies from NRC, Egypt and IECB, France. He has awarded best PhD prize in the National research Centre of Egypt (Pharmaceutical Sciences) on 2012. He has published more than 13 papers in reputed journals and has been serving as a reviewer board member of repute.
