Evaluation of long-term stability and biological activity of emulsion-based nanoadjuvants
Razim A, Gorska S, Olszewski M, Gamian A and Myc A
Polish Academy of Sciences, Poland
University of Michigan Medical School, USA
: J Clin Exp Oncol
Statement of the problem In spite of the work done by many research groups, there is still a shortage of safe and effective vaccine adjuvants on the market, especially those specialized for mucosal administration. Currently used adjuvants do not meet the needs to formulate the robust and efficacious vaccines. In response to these needs, we propose a new class of adjuvants, named as nanoadjuvants, composed of well-characterized and safe ingredients, based on an oil-in-water emulsion. Methodology: The emulsion has been characterized physicochemically and biologically using several methods. The stability of the emulsion during long-term storage (up to 12 months in 4°C, RT or 37°C) and the interaction of the emulsion with model antigen (OVA) were investigated. The cytotoxicity of the emulsion and production of proinflammatory cytokines were carefully evaluated. Findings: Several nanoadjuvants are stable up to 12 months in RT and are undamaged by sterilization (120°C, 20 min). Various nanoadjuvants can be obtained by changing the composition and amounts of ingredients. Droplet size and zeta potential of nanoadjuvants influence both physical and biological properties such as bioadhesiveness, cytotoxicity and the capacity of antigen incorporation. Conclusion & Significance: We prepared and characterized new emulsion-based formulations as a potential new class of adjuvants (nanoadjuvants) for both parenteral and mucosal vaccine administration. Our formulation may greatly contribute to development of novel vaccines.
Agnieszka Razim is a PhD student at the Institute of Immunology and Experimental Therapy Wroclaw, Poland. Her interests are mucosal vaccines and comprehensive solutions to the Clostridium difficile problem (searching for new vaccine antigens and therapeutic antibodies). She runs her own grant received from the National Science Center, Poland in which she characterizes a new emulsion-based adjuvant.
E-mail: [email protected]