Fabrication and characterizaion of biomedical materials for regenerating skin tissue using biocompatible materials from marine organisms

Seok-Chun Ko and Won-Kyo Jung

Fabrication and characterizaion of biomedical materials for regenerating skin tissue using biocompatible materials from marine organisms

Seok-Chun Ko and Won-Kyo Jung

Pukyong National University, Republic of Korea

: J Pharm Drug Deliv Res

Abstract

An emerging paradigm in wound healing techniques is that a tissue-engineered skin substitute offers an alternative approach to create functional skin tissue. Here we developed a fish collagen/alginate (FCA) sponge scaffold that was functionalized by different molecular weights of chitooligosaccharides (COSs) with the use of 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride as a cross-linking agent. The effects of cross-linking were analyzed by Fourier transform infrared spectroscopy. The results indicate that the homogeneous materials blending and cross-linking intensity were dependent on the molecular weights of COSs. The highly interconnected porous architecture with 160Ã¢Â€Â“260 m pore size and over 90% porosity and COSÃ¢Â€Â™s MW driven swelling and retention capacity, tensile property and in vitro biodegradation behavior guaranteed the FCA/COS scaffolds for skin tissue engineering application. Further improvement of these properties enhanced the cytocompatibility of all the scaffolds, especially the scaffolds containing COSs with MW in the range of 1Ã¢Â€Â“3 kDa (FCA/COS1) showed the best cytocompati-bility. These physicochemical, mechanical, and biological properties suggest that the FCA/COS1 scaffold is a superior candidate that can be used for skin tissue regeneration.