Hepatoprotective activity of the ethanolic extract of C. flammula against Paracetamol toxicity in mice
Atmani-Kilani Dina, Debbache-Benaida Nadjet, Cheraft Nassima, Tahiri Ouahiba, Sebaihi Salima, Saidene Naima and Atmani Djebbar
University of Bejaia, Algeria
: J Pharm Drug Deliv Res
Abstract
Paracetamol is widely used to treat pain and fever. However, it creates oxidative stress by causing the depletion of glutathion which leads to hepatic lesions and necrosis Clematis flammula is a plant widely used in folklore medicine in Algeria to treat inflammatory diseases. The hepatoprotective activity of this plant extracts were evaluated in vivo against paracetamol toxicity by the assessment of MDA levels superoxide dismutase and catalase activities. Histopathological analysis was equally carried out to confirm our results. Results demonstrated an increase in MDA levels (6.61±0.75 nmol/mg prot) and a decrease in the activities of superoxide dismutase (3.557±0.91 U/mg protein), catalase (2.41±0.49 U/mg protein), glutathione (2.45±0.60 μmol/mg de protéine) in paracetamol (400 mg/kg)-treated group (positive control), compared to negative control. Clematis flammula plant extracts, on the other hand, contributed at reducing MDA levels (2.34±0.47) and increasing the activities of superoxide dismutase (6.068±0.85 U/mmg protein), glutathion (17.80±2.42 μmol/mg proteins) and catalase (10.64±2.01 U/ mg protein) at 100 mg/kg. Histopathological analysis confirmed the hepatoprotective activity of this plant against paracetamol toxicity.
Biography
Atmani-Kilani Dina has completed her Master’s degree from California State University, Los Angeles and her PhD from University of Bejaia in Algeria and Postdoctoral studies from University of Bejaia. She is the Director of a project on the biological activities of Clematis flammula and is involved in two other projects on the biological activities of other local plants in Algeria. She has published more than 7 papers in reputed journals and is pursuing her research in order to identify many novel molecules in C. flammula with the collaboration of Dr. Tristan Richard in Bordeaux France and José Ignacio Ruiz Sanz in the university of the Basque country in Bilbaoa.
Email: dinaatmani88@yahoo.com
