Identification and quantification of three xanthones and two polyisoprenylated benzophenones simultaneously in fruit rind of eight Garcinia species via validated UHPLC-PDA method

<p>Premlata Kumari</p>

Identification and quantification of three xanthones and two polyisoprenylated benzophenones simultaneously in fruit rind of eight Garcinia species via validated UHPLC-PDA method

Premlata Kumari

Sardar Vallabhbhai National Institute of Technology, India

: Cell Biol (Henderson, NV)

Abstract

Statement of the Problem: Xanthones and polyisoprenylated benzophenones (PIBs) are two significant classes of plant secondary metabolites with a wide range of bioactivities. Garcinia species synthesize numerous xanthones and PIBs. Till now no method available is claiming simultaneous identification and quantification of three xanthones, α-mangostin, β-mangostin, γ-mangostin, and two PIBs, xanthochymol, isoxanthochymol.

Methodology & Theoretical Orientation: A validated ultra-HPLC (UHPLC)- photodiode array (PDA) method for the simultaneous identification and quantification of five compounds in different extracts of eight Indian Garcinia species was developed. The compounds were separated on a Waters ACQUITY™ UPLC H-Class column using a mobile phase consisting of solvents 0.1% formic acid in water (A) and methanol (B) in gradient elution mode. The total run time was 9 min.

Conclusion & Significance: From fruit rinds of eight Indian Garcinia species, namely Garcinia cambogia, G. cowa, G. indica, G. loniceroides, G. mangostana, G. morella, G. pedunculata, and G. xanthochymus, extracts were prepared using solvents of varying polarity. These extracts were analyzed for five biologically important compounds, namely α-mangostin, β-mangostin, γ-mangostin, xanthochymol, and isoxanthochymol. The results revealed that there is a wide variation in concentration of these compounds in extracts of Garcinia species. The developed and validated UHPLC-PDA method could be used for simultaneous identification and quantification of these five compounds for bioprospection of other Garcinia species.