In vivo anti-inflammatory activity by trans-resveratrol loaded-solid lipid nanoparticle for skin disorders
Roberta B Rigon, Maira Lima Gonçalez, Camila Fernanda Rodero and Marlus Chorilli
São Paulo State University, Brazil
Freie Universität Berlin, Germany
: J Pharm Drug Deliv Res
Abstract
Trans-resveratrol (RES) presents important action to prevent and treat skin disorders. Cutaneous administration of RES has being explored in order to find it on their site of action. Solid lipid nanoparticles (SLN) due to interaction characteristics with skin have being used to drug delivery system for topical application. The aim was to verify in vivo anti-inflammatory activity of SLN with RES for cutaneous application therapy. The formulations developed were composed by stearic acid (SA) as solid lipid, polysorbate 80 (P80) and soy phosphatidylcholine (SP) as surfactants and poloxamer 407 (P407) and glycerin as stabilizers (F1). Cetrimonium bromide (CB) was added as cationic surfactant to promote positive superficial charge (F2). The formulations was add of 0.25% RES (F1.RES and F2.RES). The average hydrodynamic diameters were 195.0±3.34 nm, 241.3±48.33 nm, 159.15±4.78 nm e 158.25±33.92 nm to F1, F1.RES, F2 e F2.RES, respectively. Zeta potentials (mV) were -25.5±1.01; -26.0±1.67; 30.6±1.13 e 30.0±1.85 mV for F1, F1.RES, F2 e F2.RES, respectively. Entrapment efficacy was analyzed using validated analytical methodology and both formulations (F1.RES and F2.RES) present ~50% of RES entrapped. RES solution (1:1 ethanol and water) and F2.RES presented reduction of nociception similar to dexametasone commercial cream, which suggest potent anti-inflammatory activity of RES and SLN with RES. The results demonstrated the importance to investigate the RES action when it is entrapped in drug delivery system for topical application.
Biography
Email: roberta_rigon@yahoo.com.br
