In-vitro cell compatibility of prepared collagen-glycosaminoglycans–selenium substituted hydroxyapatite nanoparticles composite scaffolds
Sara Ibrahim Korowash, A Burdzinska, E Choinska, A Abdel-Moniem Mostafa and D M Ibrahim
National Research Centre, Egypt
: J Pharm Drug Deliv Res
Abstract
Four types of scaffolds were successfully prepared by freezedrying technique: collagen-chondroitin sulfate (Co-CS), collagensodium hyalurnate (Co-SH), collagen-chondroitin sulfate-selenium substituted hydroxyapatite nanoparticles (Co-CS-SeHA2) and collagen-sodium hyalurnate-selenium substituted hydroxyapatite nanoparticles (Co-SH-SeHA2) and using gluteraldehyde as crosslinker to increase the scaffolds strength. The formed scaffolds were characterized by SEM and the results showed porous structured with desirable pore sizes ranging from 73.7 to 103 nm, for hepatocytes culture. The cell compatibility was tested using MTT test on human bone marrow mesynchymal stem cells (BM-MSCs). The best results were displayed by the scaffold (Co-CS-SeHA2). Results of fluorescent microscopy of the scaffolds showed that the highest number of cells was on Co-CS-SeHA2 scaffold. The cells were distributed in groups which suggested they proliferated on this material. The Co-CS-SeHA2 prepared scaffold can be applied for further liver/hepatocytes studies.
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