Multicomponent biomaterials for improved storing, sensing and release
Ewelina Zabost, Wioletta Liwinska, Agnieszka Kowalczyk and Zbigniew Stojek
University of Warsaw, Poland
: J Electr Eng Electron Technol
Abstract
Recent interest in designing micro and nano-sized drug delivery systems and layers is oriented on creation of delivery systems for poorly soluble and/or highly unstable substances. In our work, we would like to expand the synthesis ways and optimization steps needed for preparation of multicomponent biomaterials with improved storage, delivery and sensing properties. Firstly, we would like to present steps involved in the application of charge controlled coaxial core-shell electrospinning process for creation of thin micro and nanocomposites of PLC/PNIPA/DNA/Au NPs with attached selected anti-tumor drugs. They were attached by non-covalent and covalent bonding sensitive to tumor environment. Modified gold nanoparticles were entrapped in PLCL fibers during electrospinning process. We investigated the release profiles of drugmodified Au NPs from PLC nanofibers, by spectroscopic (UV-Vis, CD) and electrochemical techniques (CV, SWV) and in vitro experiments (HeLa, Insulinoma and Glioma cells). The morphology of composites was inspected by TEM, SEM and optical microscopy. Secondly, we would like to show several kinds of metallic NPS and hydrogel based nanoparticles modified by selected aptamers. We applied PAM and PNIPA hydrogel networks, for formation of multicomponent nanoparticles for storing of selected antitumor intercalators and releasing of it after initiation of structural changes of aptamers and volume phase transition of lattices. The DNA-based biomaterials were characterized by a strong increase in guanine and adenine anodic currents that starts at physiological temperature. The structural alterations were used as a control element in the releasing of drugs. Thirdly, we would like to expand the possibility of application of multicomponent, metallic NPS and hydrogel-based nanoparticles modified covalently with selected aptamers as sensing layers used in electrochemical sensors. We optimized the design process of such biosensors for improved detection of MUC1 protein.
Biography
