Phylogenetic study of indigenous pulses based on morphological and inter simple sequence repeat (ISSR) marker
Filemon Yusuf, Sobir and Syarif Iis Aisya
Bogor Agricultural University, Indonesia
Centre for Tropical Horticulture Studies, Indonesia
: J Plant Physiol Pathol
Abstract
Statement of the Problem: Pulses is one of the most common food consumed in Indonesia. The highest consumption rate of pulses only occurred in one single commodity, soybean. As one of the world’s richest biodiversity country, Indonesia has a high genetic diversity in pulses but some of them are still unknown. The aims of this study were: (1) determine genetic diversity of potential pulses, (2) determine the relationships among pulses and (3) characterise some pulses. Methodology & Theoretical Orientation: The study was conducted using morphological and inter simple sequence repeat (ISSR) markers among 6 accessions of winged bean (Psophocarphus tetragonolobus), 2 accessions of lima bean (Phaseolus lunatus L.), 2 accessions of Egyptian bean (Lablab purpureus), 2 accessions of cowpea (Vigna sinensis and Vigna unguiculata), 1 accessions of bambara groundnut (Vigna subterranean), 1 accession of jack bean (Canavalis ensiformis), and 1 accession of velvet bean (Mucuna pruriens). Phylogenetic tree dendogram was constructed using R program version 3.3.1. Findings: The 15 indigenous pulses have been characterised with 45 morphological characters and 8 different ISSR primers to identify genetic relationships. From the number of amplified marker alleles using ISSR primers, there are 119 polymorphic bands and none monomorphic band. Conclusion and significance: Based on morphological-molecular analysis and morphological analysis, the pulses were divided into 8 groups in dissimilarity of 20% and 32%. In molecular analysis, the pulses were divided into 7 groups in dissimilarity of 17.5%.
Biography
Filemon Yusuf is an undergraduate student at Bogor Agricultural University majoring in Agronomy and Horticulture. He has done some researches in the field of Plant Breeding. He has worked on “Effectiveness of Agrobacterium strains in transferring carotenoid gene on tobacco leaf”, during his study in Tokyo University of Agriculture and Technology as an exchange student. He has also conducted a research about in vitro mutation induction in torch ginger using gamma irradiation. He is a member of Asian Youth Network of Agriculture as the Coordinator for country of Indonesia. His projects include: “Sugar Liquid and Organic Fertilizer from Cassava Peel” in Student Creativity Program 2014 and "Orchid Entrepreneurial Development Based on Local Resources" in Student Entrepreneurial Program 2015.
