Point-of-care electrochemical cortisol sensing for health care monitoring
Ajeet Kaushik, Rahul Dev Jayant, Andrea Yndart, Venkata Atluri, Shekhar Bhansali and Madhvan Nair
Florida International University, USA
: J Electr Eng Electron Technol
Abstract
Cortisol is a potentials psychological stress biomarkers and its abnormalities due to everyday competitive life style causes many diseases. The secretion of cortisol levels found to be affected by diseases progression and substance of abuse. Thus cortisol detection and monitoring is crucial for optimization of diagnostics to decide therapeutics. In this word, for the first time, we have developed a simple, low-cost, label-free, electrochemical immunosensing platform for sensitive and selective detection of cortisol (at pM). Anti-Cortisol antibodies (Anti-Cab) immobilized self-assembled monolayer (SAM) modified microfabricated interdigitated electrodes based electrochemical cortisol in integrated with miniaturized potentiostat and a microfluidic manifold for point-of-care application. This M-P is capable to detect cortisol and exhibited a detection range from 10 pg/mL to 100 ng/mL, a detection limit of 10 pg/mL, and a sensitivity of 6 µA/(pg/mL) with the regression coefficient of 0.99. This developed electrochemical immunosensor also successfully detect saliva cortisol farm workers and plasma cortisol of HIV positive patients. Results obtained in both cases are validated with ELISA (2-5% variation). The purposed protocol can be used to correlate psychological stress of farm workers on exposure of pesticides and HIV patients with virus-infection progression parameters to optimize diagnostic and therapeutic decision.
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