Preparation and evaluation of SEDDS of metformin by in vitro, ex vivo and in vivo techniques

Zaidul I S M

Preparation and evaluation of SEDDS of metformin by in vitro, ex vivo and in vivo techniques

Zaidul I S M

International Islamic University, Malaysia

: J Pharm Drug Deliv Res

Abstract

The objective of this work was to formulate a self emulsifying drug delivery system (SEDDS) of metformin, a poorly soluble drug and to evaluate by in vivo, in vitro and ex vivo techniques. Oils and surfactants were screened out depending upon their solubilizing capacity. Among all of the solvents, Tween 80 showed very good solubilizing capacity which was 60 mg/ gm. Two excipient were used to prepare metformin SEDDS. Formulations were initially checked for the color, clarity and sedimentation. The SEDDS formulations were transparent and clear. Formulation F3 containing 1:1 (m/m) mixture of Tween 80/Capryol 90 produced smallest micro-emulsion with particles size of 11.34 ÃŽÂ¼m and drug release was higher than other formulation (101% within 20 min). Ex vivo study of the SEDDS formulation was evaluated using guinea pig intestinal sac. Drug diffused from F3 formulation was significantly higher than pure drug of metformin (p<0.001). In vivo study of SEDDS was performed in albino mice using plasma glucose level as a pharmacodynamic marker parameter. The test formulation (F3) appeared remarkable reduction in plasma glucose level, after oral administration which showed that SEDDS may be an effective technique for the oral administration of metformin.