Journal of Plant Physiology & PathologyISSN: 2329-955X

All submissions of the EM system will be redirected to Online Manuscript Submission System. Authors are requested to submit articles directly to Online Manuscript Submission System of respective journal.

Protein L-isoaspartyl (D-aspartyl) methyltransferases (PIMTs) are differentially regulated during seed development in rice and provide seed vigour and longevity


Bhanu Prakash

National Institute of Plant Genome Research, India

: J Plant Physiol Pathol

Abstract


Protein L-Isoaspartyl (D-aspartyl) methyltransferases (PIMT or PCMT EC 2.1.1.77) recognize and repair L-isoaspartate and D-aspartateresidues in damaged proteins. In dicots PIMT was shown to provide seed vigour and longevity. But the role of PIMT in during seed maturation and desiccation in orthodox seeds is largely unknown. Current study of PIMT from monocot model plant rice revealed coordinated role of PIMT isoforms during different stages of plant development and especially during seed maturation and drying. Study showed that PIMT in rice was primarily accumulated in dry seed followed by flower, leaf, stem and root. Detailed analysis of PIMT protein during seed development showed a gradual increase in its accumulation toward the end of maturation phase, retained in dry mature stage and decreased upon germination. Also we observed coordinated interplay of PIMT isoforms during seed developmental stages. Molecular analysis showed that rice codes for two PIMT genes (OsPIMT1 and OsPIMT2) which produced multiple transcripts, out of which three transcripts produced biochemically active enzymes (OsPIMT1, OsPIMT2 and ΔOsPIMT2 i.e., OsPIMT2 without N-terminal domain). We identified few biochemically inactive isoforms whose specific function is not clearly known. Ectopic expression of OsPIMTs in Arabidopsis demonstrated that all three isoforms improve seed vigour and longevity by restricting isoaspartate accumulation which was clearly evident from controlled deterioration treatments. This study elucidates in clear detail the interplay and coordinated function of various PIMTs in rice providing deeper insights into its role in germination, stress, aging and seed development especially during seed maturation and desiccation.

Biography


bhanu@nipgr.ac.in

Track Your Manuscript

Awards Nomination

GET THE APP