PTH-ANC for osteoporosis management
Zubair Jaji, Md Zuki Abu Bakar Zakaria, Rozi Mahmud, Loqman Mohamad Yusof and Mohamad Hezmee Mohamad Noor
Universiti Putra Malaysia, Malaysia
: J Pharm Drug Deliv Res
Abstract
Physicochemical and in vitro/in vivo characterization of cockle shells-derived calcium carbonate nanocrystals aragonite polymorph (ANC) as an antiosteoporotic agent and a nanocarrier in the management of osteoporosis were investigated. ANC was synthesized, using top down method, and was loaded with Human Recombinant Parathyroid Hormone (PTH 1-34) to obtain PTH-ANC conjugate. Transmission Electron Microscopy, Field Emission Scanning Electron Microscope, Zeta potential, Fourier Transmission Infrared Spectroscopy (FT-IR) X-ray diffraction spectroscopy (XRD), loading capacity, encapsulation efficiency, drug release profile, MTT and in vivo analyses were used to characterize ANC and PTH-ANC. The results demonstrated highly homogenized spherical-shaped clean aragonite nanocrystals of 30±5 nm in diameter and -27.6±8.9 mV Zeta potential. The encapsulation efficiency of ANC was directly proportional to the concentrations of the drug fed. XRD revealed strong crystallizations, no positional change of peaks was observed in PTH-ANC synthesis. FT-IR demonstrated no detectable interactions between micron aragonite and surfactant at molecular level. PTH-ANC stabilized at pH 7.5, enabling sustained slow release, and 69.83% of PTH 1-34 was released at 168 hours. 72 hour MTT relative biocompatibility study on Human Fetal Osteoblast Cell Line hFOB 1.19 revealed that ANC best supported osteoblast proliferation up to 48 hours while PTH-ANC best supported the proliferation beyond 72 hours. Weekly PTH-ANC improved bone remodeling and strength in vivo. It was concluded that PTH-ANC is a potent antiosteoporotic product for the enhancement of safety, and reduction of cost, dosage and dose frequency of PTH 1-34 due to its biogenic nature and simplicity of its synthesis.
Biography
Zubair Jaji is a Lecturer at the University of Ilorin, Ilorin, Nigeria. He has joined the University Putra Malaysia in the year 2012 as PhD student in Anatomy under the supervision of Prof MD Zuki AbuBakar.
Email: jajidvm@yahoo.com
