Synthesis, spectral characterization and theoretical studies of some biomedically potent transition metal complexes as possible photocatalyst and DNA cleaver/binder probes

A P Mishra and B S Kusmariya

Synthesis, spectral characterization and theoretical studies of some biomedically potent transition metal complexes as possible photocatalyst and DNA cleaver/binder probes

A P Mishra and B S Kusmariya

Dr. Hari Singh Gour Central University, India

: J Pharm Drug Deliv Res

Abstract

Synthesis and structural characterization of new coordination complexes has always been a challenging task to the chemists. 3d-metal complexes derived from Schiff bases and their derivatives have been studied extensively due to their facile synthesis, unusual configurations, and structural labiality. The development of such types of complexes as multifunctional materials represents one of the main targets for their potential applications in photocatalyst and DNA clever/binder agents. The Schiff base ligands and their complexes have been synthesized by reported refluxing/condensation method. To establish and confirm the chemical structure of synthesized products, various techniques have been employed viz. FTIR, 1H-NMR, mass spectrometry, electronic, CV, thermal, XRD, SEM-EDX and single crystal X-ray diffraction studies. The useful information about molecular structure and related properties of the synthesized compounds has been produced at B3LYP level using density functional theory. Such complexes have been used as a precursor to fabricate metal oxide nanoparticles by pyrolytic method. The nanoparticles have been characterized by powder X-ray diffraction, SEM, TEM, FT-IR spectroscopy and UV-Vis spectroscopy techniques. The visible light driven photocatalytic activity of prepared nanoparticles has been demonstrated using methylene blue (MB) as a representative dye. DNA offers several potential binding sites for transition metals, including the anionic phosphate backbone, electron-rich bases, and the major or minor grooves. The interaction of synthesized complexes with calf thymus DNA (ct DNA) has been investigated in vitro using UV-Vis, fluorescence, CV and gel electrophoresis techniques. The calculated binding constant and site size binding shows the interactive model between complexes and ct DNA. The interaction between plasmid DNA (pTZ57R DNA) and these complexes is confirmed by gel electrophoresis studies. The computer-aided molecular docking techniques have also been carried out to ascertain the mode of action toward the molecular target DNA for selected ligand and complexes.