Targeted 13C metabolic tracer fate association study and regression analysis reveal diverse cellular phenotypes
László G Boros
Harbor-UCLA Medical Center, USA
: J Diagnos Tech Biomed Anal
Abstract
Regression statistics in this targeted tracer fate association study (TTFAS) is shown to reveal associations among diverse phenotypic metabolic products in fumarate hydratase-deficient UOK kidney tumor cells with defective glutaminolysis, reductive carboxylation for lipogenic citrate production, as well as the Warburg effect, using the [1,2-13C2]-D-glucose tracer. These co-existing profiles were revealed in previous 13C-glutamine and 13C-glucose tracer experiments. Herein UOK cells show a close Warburgtype correlation between consumption of glucose for 13C-lactic acid production (R2>0.98; glucose to lactate). On the other hand, proliferation-related macromolecule 13C labeling, such as that of RNA-derived ribose and lignoceric acid, correlates with the glucosederived internally cross-labeled 13C-glutamine fraction (R2>0.98; glutamate to lignocerate and RNA ribose). The TTFAS approach reliably re-produces results and conclusions obtained via multiple 13C-tracer metabolic flux analyses using a single metabolic tracer to trim down versatilities, cost and time, involved in multiple metabolic tracer studies using 13C and deuterium as labeling atoms for diverse products.
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