Journal of Pharmaceutics & Drug Delivery Research .ISSN: 2325-9604

All submissions of the EM system will be redirected to Online Manuscript Submission System. Authors are requested to submit articles directly to Online Manuscript Submission System of respective journal.

Topical drug delivery and nanotoxicity as revealed by advanced fluorescence microscopy

World Congress on Drug Delivery, Formulation and Analytical Techniques

July 02-03, 2018 New Orleans, USA


Ulrike Alexiev

Freie Universitat Berlin, Germany

ScientificTracks Abstracts : J Pharm Drug Deliv Res
DOI : 10.4172/2325-9604-C4-035

Abstract


Fluorescence Lifetime Imaging Microscopy (FLIM) provides new possibilities in the investigation of nanocarrier penetration and drug delivery, as the fluorescence lifetime is highly sensitive to the microenvironment. Using Cluster-FLIM, image contrast is enhanced and a clear-cut discrimination between different fluorescent species is feasible. This allows visualization of nanoparticles with an unprecedented precision and sensitivity in skin sections, skin biopsis, living cells and in living tissue. The latter is realized in a multiphoton FLIM setup. To further increase resolution and to resolve nanocarrier fine-structure we employed single molecule TIRF microscopy. Using this method, we were also able to show the penetration of individual nanoparticles into the viable epidermis, thereby adding new information to the debate of whether nanoparticles can cross the skin barrier. Since nanotoxicity is a major concern in the application of nanoparticles, we recently developed a novel FLIM-based imaging assay (FLIM-ROX) for reactive oxygen species detection. Using FLIM-ROX, we demonstrate the nanotoxicity of subcytotoxic amounts of nanoparticles by linking cellular adverse effects to low-level oxidative stress.

Biography


Ulrike Alexiev fields of expertise are molecular and cellular biophysics and analytical nanomedicine, in particular in the area of experimental dermatology and topical drug delivery.

E-mail: [email protected]

Track Your Manuscript

Recommended Conferences

Share This Page