UPLC-MS/MS method for the determination of Vilazodone in human plasma: Application to a pharmacokinetic study
Marwa Fouad, Ramzia El-Bagary, Hanaa Hashem and Sally Tarek
Cairo University, Egypt
: J Diagnos Tech Biomed Anal
Abstract
A sensitive, rapid and simple liquid chromatography–electrospray ionization mass spectrometry (LC-ESI-MS/MS) method was developed for the quantitative determination of vilazodone in human plasma and to study the pharmacokinetic behavior of vilazodone in healthy Egyptian volunteers. With escitalopram as internal standard (IS), liquid/liquid extraction was used for the purification and pre-concentration of analytes from human plasma matrix using diethyl ether. The separation was performed on Acquity UPLC BEH shield RP C18 column (1.7 um, 2.1x150 mm). Isocratic elution was applied using methanol: 0.2% formic acid (90:10, v/v). Detection was performed on a triple-quadrupole tandem mass spectrometer with multiple reactions monitoring (MRM) mode via electrospray ionization (ESI) source at m/z 442.21 → 155.23 for vilazodone and m/z 325.14 → 109.2 for escitalopram. Linear calibration curves were obtained over the range of 1-200 ng/mL with the lower limit of quantification at 1 ng/mL. The intra- and inter-day precision showed relative standard deviation ≤ 3.28%. The total run time was 1.5 min. This method was successfully applied for clinical pharmacokinetic investigation and preliminary metabolic study was carried out.
Biography
Marwa Fouad has completed her PhD in 2009 from Faculty of Pharmacy, Cairo University and her Postdoctoral studies from UMR-MD1 Unit, Aix-Marseille University, France in 2013 and from Pharmaceutical Analysis Laboratory, Mons University, Belgium in 2014. She is currently the director of Quality Assurance Unit in the Center of Applied Research and Advanced Studies (CARAS) at the Faculty of Pharmacy, Cairo University. She has published more than 15 papers in reputed journals and is serving as a peer reviewer for many reputed journals.
