Variant response of Liao, Aao, and protease during in vitro culture of Argyrolobiumroseum

Muhammad Zia

Variant response of Liao, Aao, and protease during in vitro culture of Argyrolobiumroseum

Muhammad Zia

University of Haripur Khayber Pakhtunkhwa, Pakistan

: J Plant Physiol Pathol

Abstract

I ndole acetic acid oxidase (IAAO), ascorbic acid oxidase (AAO), and protease were analyzed during in vitro tissue culture of Argyrolobium roseum. These enzymes are crucial for biochemical regulation for growth and developmental processes. The enzymes were studied during growth from seed; callus induction from different explants; organogenesis period; and acclimatization conditions. It was observed that leaf derived calli presented 23.7% and 29.4% increase in IAAO and protease enzyme, respectively as compared to stem (16.1% and 91%) and root (17.1% and 23%, respectively). The decline level in Ascorbic acid oxidase was observed during in vitro conditions.Calliproduced fromleaf showed 18.47% decline in AAO level, whereas stem showed 12.5% and root 22.4%. Behavior of IAAO, AAO and protease changed during organ genic phase. Root accessible 19.3% decrease in IAAO level as compared to regenerated stem (31.8%) and leaf (29.9%), however protease showed 28.6% decline in regenerated leaf as compared to stem (26.4%) and root (31.7%). Regenerated leaves showed 39.57% increase in AAO enzyme level. Similarhigher pattern was observed in stem (30%) and root (47%). The study highlights the biochemical behavior of IAAO, AAO and protease during in vitro life pattern of A. roseum.