Andrology & Gynecology: Current ResearchISSN: 2327-4360

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Research Article, Androl Gynecol Curr Res Vol: 1 Issue: 3

Role of Escherichia coli Pili Adhesion Molecule to Inhibit Escherichia coli Adhesion to Human Spermatozoa In vitro

Sukarjati1*, Doddy M Soebadi2 and Aucky Hinting3
1Department of Biology, School of Science and Mathematics, PGRI Adi Buana University, Surabaya, Indonesia
2Department of Urology, Airlangga University School of Medicine, Surabaya, Indonesia
3Department of Medical Biology, Airlangga University School of Medicine, Surabaya, Indonesia
Corresponding author : Dr. Sukarjati
Department of Biology, School of Science and Mathematics, PGRI Adi Buana University, Surabaya, Indonesia
Tel: 031-504-1097; Fax: 031-505-3468; E-mail: [email protected]
Received: October 09, 2014 Accepted: November 19, 2014 Published: November 21, 2014
Citation: Sukarjati, Soebadi DM, Hinting A (2013) Role of Escherichia coli Pili Adhesion Molecule to Inhibit Escherichia coli Adhesion to Human Spermatozoa In vitro. Androl Gynecol: Curr Res 1:3. doi:10.4172/2327-4360.1000107

Abstract

Role of Escherichia coli Pili Adhesion Molecule to Inhibit Escherichia coli Adhesion to Human Spermatozoa In vitro

To investigate capacity of adhesin molecule to inhibit the adhesion of Escherichia coli (E. coli) to human spermatozoa in vitro. E. coli was obtained by culturing the semen of infertile males. Spermatozoa were taken from healthy donors with normal spermatozoa according to WHO criteria (1999). Pili cutting were performed five times gradually using omnimixer. To find the factions of pili and protein with highest hemagglutinin protein titer, we performed hemagglutination test. Protein isolation was performed using SDS PAGE electrophoresis, electroelusion, dialysis and precipitation. Protein concentration estimation was conducted using Biuret assay method. Spermatozoa were prepared with Sil Select Plus.

Keywords: E. coli; Human spermatozoa; Adhesin molecule

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