Journal of Otology & RhinologyISSN: 2324-8785

All submissions of the EM system will be redirected to Online Manuscript Submission System. Authors are requested to submit articles directly to Online Manuscript Submission System of respective journal.
bahis siteleri bahis siteleri bahis siteleri casino siteleri

Promoter Methylation of Dual Specificity Phosphatase-6 Gene effect on Invasion and Metastasis of Nasopharyngeal Carcinoma

Objective: The dual-specificity phosphatase-6 (DUSP-6) is a member of dual-specificity protein phosphatase (DUSP) family, it can make residues of threonine and tyrosine dephosphorylate. In recent years, it was found that DUSP-6 is correlated with tumour generation and progress. In this study we have detected the effect of DUSP-6 gene methylation on invasion and metastasis of nasopharyngeal carcinoma.

Methods: RT-PCR and methylation-specific PCR are used to detect mRNA expression and methylation state of DUSP-6 gene in nasopharyngeal carcinoma tissues and CNE2 cell line. The changes of mRNA expression and methylation state of DUSP-6 gene, proliferation and invasion of cells were detected after 5-aza- 2’-deoxycytidine (5-aza-cdR) treated CNE2 cell line.

Results: These results showed that DUSP-6 gene expression is related to its promoter methylation state, cervical lymph node metastasis is related to DUSP-6 gene promoter methylation state in patients with nasopharyngeal carcinoma, 5-aza-cdR can inhibit invasion of CNE2 cells, and make re-expression of DUSP-6 gene by promoter demethylation in CNE2 cells.

Conclusion: DUSP-6 gene promoter methylation state is possibly an important factor that effected invasion and metastasis of nasopharyngeal carcinoma. DUSP-6 gene may make a novel marker for prognostic evaluation in nasopharyngeal carcinoma.

 

Special Features

Full Text

View

Track Your Manuscript

Media Partners

GET THE APP