Screening of invitro activity of antimicrobial and antibiofilm property of Mesenchymal stems cells against MDR gram-negative organism isolated from urinary tract infections in tertiary care hospital
Recent studies had shown that Mesenchymal Stem Cells (MSCs) have beneficial effects on bacterial infections. Treatment with MSCs has proven bacterial clearance. This study was undertaken to study the in vitro activity of antimicrobial and antibiofilm activity of stems cells against gram-negative multidrug-resistant organisms from urinary tract infections. The samples will be processed according to standard protocol following standard guidelines. All the isolates obtained will be identified by standard guidelines. A total of 50 isolates was collected.
The antibiotic susceptibility testing will be done for all the isolates by Kirby Bauer disc diffusion method following CLSI guidelines. All the isolates are screened for the production of biofilm by the tissue culture plate method. The antimicrobial activity of mesenchymal stem cells was done by the micro broth dilution method.
Among the 50 gram-negative isolates 22 (44%) were Pseudomonas species 12 (24%) were E coli 8 (16%) were Klebsiella spp and 8 (16%) were Proteus species.
Among the 50 isolates, 32 (64%) were multi-drug resistant to the antibiotics tested. Among the 50 isolates, 43 (86%) produced biofilm of which 28 (65%) were strong producers 8 (18%) were moderate biofilm producers and 7(16.27%) were weak biofilm producers. All 43 isolates showed sensitivity for the mesenchymal stem cells with a MIC range of 32-0.25? g.
So far only very few or no studies have been reported on the anti-biofilm activity of mesenchymal stems cells. From our study stem cell, therapy with MSC will be effective and alternate for antibiotic resistance in chronic urinary infection thereby can serve as a therapeutic option for treating drug-resistant organisms.