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Journal of Veterinary Science & Medical Diagnosis ISSN: 2325-9590

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Research Article, J Vet Sci Med Diagn Vol: 3 Issue: 2

Analysis of Virulence Associated and Antibiotic Resistance Genes of Microbes in Subclinical Mastitis Affected Cattle Milk by Pyrosequencing Approach

Vaibhav D. Bhatt1, Anju P. Kunjadia2*, Keyur D. Bhatt3, Navin R. Sheth1 and Chaitanya G. Joshi4
1Department of Pharmaceutical Sciences, Saurashtra University, Rajkot-360 005, Gujarat, India
2Ashok and Rita Patel Institute of Integrated Study and Research in Biotechnology and Allied Sciences, ADIT campus, New Vallabh Vidyanagar -388 120, Anand, Gujarat, India
3Department of Chemistry, Gujarat University, Ahmedabad, Gujarat, India
4Department of Animal Biotechnology, College of Veterinary Science and A. H., Anand Agricultural University, Anand-388 001, Gujarat, India
Corresponding author : Dr. Anju P. Kunjadia
Associate Professor, Ashok and Rita Patel Institute of Integrated Study and Research in Biotechnology and Allied Sciences, ADIT campus, New Vallabh Vidyanagar -388 120, Anand, Gujarat, India
E-mail: [email protected]
Received: February 06, 2014 Accepted: April 03, 2014 Published: April 08, 2014
Citation: Vaibhav DB, Anju PK, Keyur DB, Navin RS, Chaitanya GJ (2014) Analysis of Virulence Associated and Antibiotic Resistance Genes of Microbes in Subclinical Mastitis Affected Cattle Milk by Pyrosequencing Approach. J Vet Sci Med Diagn 3:2. doi:10.4172/2325-9590.1000134

Abstract

Analysis of Virulence Associated and Antibiotic Resistance Genes of Microbes in Subclinical Mastitis Affected Cattle Milk by Pyrosequencing Approach

Metagenomic analysis of milk samples collected from Gir, Kankrej (Bos indicus) and crossbred (Bos taurus X Bos indicus) cattle harbouring subclinical mastitis was carried out by pyrosequencing technology to elucidate virulence associated genes and sequences from the microbial community. Metabolic profile by a web-based tool MG-RAST revealed that members of Virulence, disease and defense associated genes were found to be 3.53,2.88 and 5.26 per cent (the percent values were calculated by finding its proportional hits among total hits obtained) in Gir, Kankrej and crossbred cattle, respectively.

Keywords: Metagenomic; Virulence; MG-RAST; Bacteriocins; Superantigens

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