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Hindi VEGETOS: An International Journal of Plant ResearchOnline ISSN: 2229-4473
Print ISSN: 0970-4078
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Alok Kumar Author
Publications
Research Article Subscription
Selection of Culture Media and Optimization of Media Composition for Extracellular Production of Lipase by Acinetobacter sp. AH-19
Author(s): Alok Kumar, Hari Ram, Lebin Thomas and Ved Pal Singh
Selection of Culture Media and Optimization of Media Composition for Extracellular Production of Lipase by Acinetobacter sp. AH-19
A total of 20 strains of bacteria were isolated from cow shade area soil collected in India. The isolated strains were screened using Rhodamine B dye on tributyrin containing nutrient agar petriplates. One isolated strain, exhibiting a broader clear zone than the remaining ones, indicating its higher lipase activity, was selected and identified as Acinetobacter sp. AH-19 based on morphological and biochemical characterization. Out of the four culture media (MI to M-IV) tested, the maximum production (78 U/ml) of lipase was observed in M-IV, th... view more»