Cell Biology: Research & TherapyISSN: 2324-9293

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Aruna V Vanikar Author

Subjects of specialization
Cellular Immunology,Cellular Pathology,Cell Regeneration,Diagnostics,Medicine

Affiliation
Department of Cell therapy and Regenerative Medicine, Gujarat, India

Biography

Department of Cell therapy and Regenerative Medicine, G.R. Doshi and K.M. Mehta Institute of Kidney Diseases &Research Centre (IKDRC)- Dr. H.L. Trivedi Institute of Transplantation Sciences (ITS)


Publications

Research Article Subscription

Comparison of Alpha Modified Eagle’s Minimum Essential Medium with Dulbecco’s Modified Eagle’s Medium For In-Vitro Generation of Human Adipose Tissue Derived Mesenchymal Stem Cells.

Author(s):

Chetan N Patel, Aruna V Vanikar*, Kunal S Gupte, Jignesh V Patel and Lovelesh A Nigam

Background: Alpha modified Eagle’s minimum essential medium (α-MEM) and Dulbecco’s modified Eagle’s medium (DMEM) are the basic media for cell culture. α-MEM has minimum glucose and amino acid concentration than DMEM. We report a study comparing the individual effect of these two media used for generation of adipose tissue derived mesenchymal stem cells (ADMSC), in terms of quantity, viability and immunophenotyping. Material and Methods: Ten grams adipose tissue resected from anterior abdominal wall of 5 willing healthy donors was divided equally into two 75 cm2 tissue culture flasks with 40 ml culture medium, one containing α-MEM and the other containing DMEM. Adipose tissue was minced into tiny pieces, incubated in collagenase-I at ... view more»

DOI: 10.4172/2324-9293.1000140

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