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Hindi Cell Biology: Research & TherapyISSN: 2324-9293
Bhargav AG Author
Subjects of specialization
Affiliation
Cellular Immunology,Metabolomics,Cell Tissue Studies,treatment of diseases,Cell Motility
Department of Pathology, Laboratory Medicine, Transfusion Services and Immunohematology
Biography
Department of Pathology, Laboratory Medicine, Transfusion Services and Immunohematology. G.R. Doshi and K.M. Mehta Institute of Kidney Diseases and Research Centre (IKDRC) - Dr. H.L. Trivedi Institute of Transplantation Sciences (ITS), India
Publications
Research Article Subscription
Optimization of In vitro Culture Conditions of Interleukin-10 Secreting B Regulatory Cells of Human Origin
Author(s): Gupte KS, Vanikar AV, Patel CN, Bhargav AG, Patel JV and Thakkar UG
Abstract
Aim: B-regulatory cells (B-regs), commonly characterized as CD19+/38hi/24hi, comprise of a major subset of IL-10 secreting B-cells. We report optimization of culture conditions for B-regs of human origin, obtained from in-vitro co-culture of donor adipose tissue derived mesenchymal stem cells (AD-MSC) and renal allograft recipient (RAR) peripheral blood mononuclear cells (PBMC) for potential cell therapy.
Material and methodology: Five sets of 6-well plates were filled with proliferation media. Each plate was seeded with 5 × 104 ADMSC and 1 × 106 cells of responder and stimulator peripheral blood mononuclear cells (PBMC) and incubated at 37ËšC, 5% CO2 after addition of lipopolysaccharide–E.coli-K12 strain (LPSEK) in varying concentrations. At each ... view more»
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