Spanish 
Chinese 
Russian 
German 
French 
Japanese 
Portuguese 
Hindi Journal of Blood Research & Hematologic Diseases
Chethana Ramachandraiah Author
Subjects of specialization
Affiliation
Pisum Sativum pod extract, Anticoagulant; Antiplatelet, Serine protease, Fibrinogenolysis
Department of Studies and Research in Biochemistry and Centre for Bioscience and Innovation, Tumkur University, Tumkur, Karnataka, India
Biography
Chethana Ramachandraiah evaluates the anticoagulant and antiplatelet activities of Pisum sativum pod extract (PSPE). PSPE revealed similar protein banding pattern under both reducing and nonreducing conditions on SDS-PAGE, suggesting the presence of monomeric proteins in the extract. PSPE showed proteolytic activity as it hydrolyzed casein and gelatin and the specific activity was found to be 0.16 and 0.21 units/mg/min respectively
Publications
Research Article Subscription
Evaluation of Anticoagulant and Antiplatelet Activity of Pisum sativum Pod Extract
Author(s): Chethana Ramachandraiah, Sharath Kumar M Nandish, Jayanna Kengaiah, Ashwini Shivaiah, Chandramma, Kesturu S.Girish, Kempaiah Kemparaju and Devaraja Sannaningaiah
The current study evaluates the anticoagulant and antiplatelet activities of Pisum sativum pod extract (PSPE). PSPE revealed similar protein banding pattern under both reducing and nonreducing conditions on SDS-PAGE, suggesting the presence of monomeric proteins in the extract. PSPE showed proteolytic activity as it hydrolyzed casein and gelatin and the specific activity was found to be 0.16 and 0.21 units/mg/min respectively. The proteolytic activity of PSPE was completely abolished by PMSF but not IAA, EDTA and 1,10, Phenanthroline suggesting the presence of serine protease. Further, PSPE was evaluated for anticoagulation efficiency. Interestingly, PSPE increased the clotting time of citrated human plasma from the control 220 sec to 460 sec suggesting its ... view more»
Google Scholars / Researchers Sites
