Journal of Blood Research & Hematologic Diseases

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Research Article, J Blood Res Hematol Dis Vol: 2 Issue: 1

Evaluation of Anticoagulant and Antiplatelet Activity of Pisum sativum Pod Extract

Chethana Ramachandraiah1, Sharath Kumar M Nandish1, Jayanna Kengaiah1, Ashwini Shivaiah1, Chandramma1, Kesturu S.Girish1, Kempaiah Kemparaju2 and Devaraja Sannaningaiah1*

1Department of Studies and Research in Biochemistry and Centre for Bioscience and Innovation, Tumkur University, Tumkur, Karnataka, India

2Department of Studies in Biochemistry, University of Mysore, Manasagangothri, Mysore, Karnataka, India

*Corresponding Author : Devaraja Sannaningaiah, Ph.D.
Assistant Professor, Department of Studies and Research in Biochemistry, Tumkur University, BH Road, Tumkur-572103, India
Tel: +91-9902838928
E-mail: [email protected]

Received: October 19, 2016 Accepted: November 03, 2017 Published: November 08, 2017

Citation: Ramachandraiah C, Nandish SKM, Kengaiah J, Shivaiah A, Chandramma, et al. (2017) Evaluation of Anticoagulant and Antiplatelet Activity of Pisum sativum Pod Extract. J Blood Res Hematol Dis 2:1.

Abstract

The current study evaluates the anticoagulant and antiplatelet activities of Pisum sativum pod extract (PSPE). PSPE revealed similar protein banding pattern under both reducing and nonreducing conditions on SDS-PAGE, suggesting the presence of monomeric proteins in the extract. PSPE showed proteolytic activity as it hydrolyzed casein and gelatin and the specific activity was found to be 0.16 and 0.21 units/mg/min respectively. The proteolytic activity of PSPE was completely abolished by PMSF but not IAA, EDTA and 1,10, Phenanthroline suggesting the presence of serine protease. Further, PSPE was evaluated for anticoagulation efficiency. Interestingly, PSPE increased the clotting time of citrated human plasma from the control 220 sec to 460 sec suggesting its anticoagulant property. Furthermore, PSPE specifically prolonged the clotting time of APTT but not PT revealed the interference in intrinsic pathway of coagulation cascade. In addition, PSPE exhibited anti-aggregation activity by inhibiting agonists such as ADP and epinephrine induced platelet aggregation in a dose dependent manner and the detected aggregation inhibition was found to be 58% and 82% respectively. In addition, PSPE preferentially hydrolyzed Aα and Bβ chain of human fibrinogen and all the chains of fibrin clot. Fibrinogenolytic and fibrinolytic activity of PSPE was inhibited by PMSF but not IAA, EDTA and 1,10, Phenanthroline suggesting the presence of serine protease in the extract. Moreover, PSPE did not hydrolyze RBC cells signifying its non-toxic property.

Keywords: Pisum Sativum pod extract; Serine protease; Anticoagulant; Antiplatelet; Fibrinogenolysis

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