Daihachiro Tomotsune Author

Research Article Open Access

Simulation of Embryonic Environments Produced by the Effect of Estradiol on Induced Pluripotent Stem Cells

Author(s):

Sawako Sakai, Daihachiro Tomotsune, Kanji Hirashima, Sakiko Takizawa, Tadayuki Yokoyama, Fengming Yue, Rika Tani, Ken Matsumoto and Katsunori Sasaki

Estradiol (E2) is one of the sex steroid hormones, whose various well-known functions include control of the female reproductive system, causing breast cancers, and inhibiting arterial sclerosis. In this study to investigate how E2 affected cell differentiation of induced pluripotent stem cells (iPSCs), we used different methods to culture hiPSCs, the undifferentiated state, longterm culture without passages (natural differentiation), embryoid bodies (EBs), and accelerated differentiation by activin, focusing on marker gene expression, including Brachyury, hepatocyte nuclear factor (HNF) 3β, estrogen receptor α, and other markers. When iPSCs began to differentiate by responding to E2, Brachyury expression decreased regardless of the amount of E2, ... view more»

DOI: 10.4172/2325-9620.1000144

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