Dominique Vanpeeand Laurence Galanti Author
Subjects of specialization
Affiliation
Cotinine, QDa, supported liquid extraction, ultra-high performance liquid chromatography, quantification method development
IRSS, 30 Clos Chapelle-aux-champs, 1200 Woluwe-Saint-Lambert, Belgium
Myself Dominique Vanpeeand Laurence Galanti , I belong to IRSS, 30 Clos Chapelle-aux-champs, 1200 Woluwe-Saint-Lambert, Belgium |
Research Article Open Access
Author(s): Marie-Lise Colsoul, Nicolas Goderniaux, Dominique Vanpee and Laurence Galanti
Objective: Cotinine is the best biomarker of tobacco smoke exposure because of its long half-life. Several methods are developed to quantify cotinine in biological fluids, including high performance liquid chromatography. This method requires an extraction step in order to clean-up the sample and concentrate the analyte of interest. The aim of this study was to optimise the extraction step and to develop an easy, reproducible and specific method to measure cotinine in urine. Methods: An extraction of neutrals was chosen and performed by Supported Liquid Extraction (SLE). SLE consists in liquidliquid extraction in the presence of a sorbent enabling efficient extraction with less organic solvent and without any emulsion formation. Urine was basified by treatment with NH4OH in order to neu... view moreĀ»