Elias AL Tawil Author

Research Article Open Access

Biomimetic Three Dimensional Cell Culturing: Colorectal Cancer Micro-Tissue Engineering

Author(s):

Yusra L Kassim, Elias AL Tawil, Didier Lecerf, J?r?me Couteau, Thomas Simon, Catherine Buquet, Jean Pierre Vannier and Elise Demange

Background: Spheroid cultures are known to mimic closely the properties of tumor tissue than monolayer cultures with regard to growth kinetics and metabolic rates. The aim of this paper is to confirm that tumor micro-tissue in a 3D biocompatible microenvironment maintain the cells natural behavior when compared to 2D monolayer culturing.

Method: In order to validate our 3D culture system, we compared the 3D culture within a cross-linked hydrogel of hyaluronic acid, one of the major components of the extracellular matrix and the conventional 2D culture system.

Results: Interestingly within our culture system, cells could be analyzed eit... view more»

DOI: 10.4172/2324-9110.1000123

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